BCH210H1

U of T

Course Overview

Grade Boosters

Lessons & Practice

1. Fundamental Interactions

44min

1.1.1. From Monomers to Polymers4 min

1.2.1. Unique Properties of Water6 min

1.2.2. Practice 22 min

1.3.1. Types of Chemical Bonds 5 min

1.3.2. Practice 1

1.3.3. Practice 2

1.4.1. Intermolecular Forces: Hydrogen Bonding5 min

1.4.2. Example 2 min

1.4.3. Practice 12 min

1.4.4. Practice 22 min

1.4.5. Practice 33 min

1.5.1. Intermolecular Forces: Van der Waals Interactions 11 min

1.5.2. Practice 13 min

1.5.3. Practice 2

1.Q. Checkpoint Quiz5 Qns

2. pH and Buffers

35min

2.1.1. Acids-Base Definitions, pH, pKa5 min

2.2.1. pKa and Ionization State8 min

2.2.2. pKa of Amino Acids10 min

2.2.3. Amino Acid Isoelectric Point5 min

2.2.4. Practice: pKa of Side Chains4 min

2.3.1. Buffers4 min

2.Q. Checkpoint Quiz4 Qns

3. Amino Acid Structures

1.2hr

3.1.1. Peptide Bond6 min

3.1.2. Peptide Bond Angles3 min

3.1.3. Example: Peptide Chain4 min

3.1.4. Example: Peptide Bond Angles2 min

3.1.5. Practice: Peptide Bond Resonance2 min

3.2.1. Primary and Secondary Protein Structure5 min

3.2.2. Example: Point Mutation in Secondary Structure3 min

3.3.1. α-helix3 min

3.3.2. Properties of α-helix5 min

3.3.3. Example: Properties of an Alpha Helix3 min

3.3.4. Example: Point Mutation in Alpha Helix4 min

3.3.5. Practice: Alpha-Helix3 min

3.4.1. β-Sheet4 min

3.4.2. β-turns and loops5 min

3.4.3. Example: Beta-Turns2 min

3.4.4. Example: H-bonding in Beta-Sheets2 min

3.4.5. Example: Beta-Barrel Channels4 min

3.5.1. Tertiary and Quarternary Protein Structure9 min

3.5.2. Example: Tertiary Structure (Motifs)3 min

3.5.3. Practice: Quaternary Structure3 min

3.Q. Checkpoint Quiz6 Qns

4. Amino Acid Properties

1.3hr

4.1.1. Protein Functions3 min

4.2.1. Amino Acids10 min

4.2.2. Example: Similar Amino Acids3 min

4.2.3. Example: Directed Mutagenesis3 min

4.3.1. Hydrogen Bonds4 min

4.3.2. Hydrophobic Interactions4 min

4.3.3. Electrostatic/Ionic Interactions3 min

4.3.4. Van der Waals Forces3 min

4.3.5. Disulfide Bond3 min

4.3.6. Example: Hydrogen Bonding3 min

4.3.7. Example: Non-Covalent Bonds4 min

4.3.8. Practice: Amino Acid Hydrophobicity5 min

4.4.1. Acids-Base Definitions, pH, pKa5 min

4.4.2. pKa and Ionization State8 min

4.4.3. pKa of Amino Acids10 min

4.4.4. Amino Acid Isoelectric Point5 min

4.4.5. Practice: pKa of Side Chains4 min

4.Q. Checkpoint Quiz4 Qns

5. Protein Structure and Folding

20min

5.1.1. Anfinsen's Experiment8 min

5.1.2. Thermodynamics of Protein Folding7 min

5.1.3. Example: Urea2 min

5.1.4. Practice: Protein Folding2 min

5.Q. Checkpoint Quiz3 Qns

6. Protein Purification and Identification

36min

6.1.1. Chromatography7 min

6.1.2. Chromatography: Part II7 min

6.1.3. SDS-PAGE (Electrophoresis)6 min

6.2.1. Methods of Determining Protein Sequence3 min

6.2.2. Protein Detection Techniques6 min

6.2.3. Mass Spectrometry2 min

6.2.4. Practice: Peptide Digest6 min

6.Q. Checkpoint Quiz4 Qns

7. Studying Protein Structure

37min

7.1.1. Predicting Protein Stucture3 min

7.1.2. Chou-Fasman Rules5 min

7.1.3. Applications of Chou-Fasman Rules5 min

7.1.4. Example: Predicting Tertiary Structure2 min

7.1.5. Example: Chou-Fasman Rules2 min

7.1.6. Practice: Predicting Structure from Chou-Fasman Parameters3 min

7.2.1. X-ray Crystallography2 min

7.2.2. Electron Microscopy2 min

7.2.3. Nuclear magnetic resonance (NMR)4 min

7.2.4. Infrared spectroscopy (IR)1 min

7.2.5. Circular Dichroism (CD)3 min

7.2.6. Example: Methods for Determining Protein Structure45 sec

7.2.7. Example: Determining Structure of Small Protein1 min

7.2.8. Practice: Match the Protein to the Technique2 min

7.2.9. Example: Affinity Tag Chromatography2 min

7.Q. Checkpoint Quiz4 Qns

8. Functional Studies

14min

8.1.1. Pulldown Assays and Crosslinking6 min

8.1.2. ReporterAssays7 min

8.1.3. Example: Fluorescence Microscopy1 min

8.Q. Checkpoint Quiz1 Qn

9. Enzymes + Kinetics

13min

9.1.1. Enzyme Kinetics3 min

9.1.2. Michaelis-Menten Equation5 min

9.1.3. Lineweaver-Burke Plot2 min

9.1.4. Example: Lineweaver Burke Plot1 min

9.1.5. Enzyme Kinetics Practice Question2 min

9.Q. Checkpoint Quiz4 Qns

10. Kinetics (cont.)

44min

10.1.1. Reversible/Irreversible Inhibition3 min

10.1.2. Competitive Inhibition2 min

10.1.3. Non-Competitive Inhibition3 min

10.1.4. Example: Enzyme Inhibition2 min

10.1.5. Cheatsheet: Summary of Inhibitors

10.2.1. Enzyme Regulation3 min

10.2.2. Ligand Binding10 min

10.2.3. Hemoglobin10 min

10.2.4. Dissorders in Hemoglobin2 min

10.2.5. Chymotrypsin7 min

10.2.6. Chymotrypsin2 min

10.Q. Checkpoint Quiz4 Qns

11. Biological Lipids and Membranes

56min

11.1.1. Introduction to Lipids2 min

11.1.2. Fatty Acids7 min

11.1.3. Triacylglycerols2 min

11.1.4. Glycerophospholipids3 min

11.1.5. Sphingolipids2 min

11.1.6. Cholesterol3 min

11.1.7. Signalling Lipids (i.e., Steroids)3 min

11.1.8. Example: Naming Fatty Acids3 min

11.1.9. Example: Identifying Lipids1 min

11.1.10. Practice: Fatty Acids1 min

11.2.1. The Lipid Bilayer6 min

11.2.2. Membrane Lipid Assembly3 min

11.2.3. Membrane Fluidity8 min

11.2.4. Membrane Lipid Dynamics2 min

11.2.5. Membrane Analysis6 min

11.2.6. Example: Membrane Fluidity1 min

11.2.7. Example: Cellular Membranes2 min

11.2.8. Practice: Transverse Diffusion of Lipids1 min

11.Q. Checkpoint Quiz5 Qns

12. Membrane Protein Structure

25min

12.1.1. Proteins in the Lipid Bilayer1 min

12.1.2. Peripheral Membrane Proteins5 min

12.1.3. Integral Membrane Proteins5 min

12.1.4. Measuring Hydrophobicity7 min

12.1.5. Membrane Protein Folding2 min

12.1.6. Practice: Peripheral Membrane Proteins1 min

12.1.7. Practice: Integral Membrane Proteins1 min

12.1.8. Practice: Hydropathy49 sec

12.1.9. Practice: Integral Membrane Proteins55 sec

13. Membrane Protein Function

51min

13.1.1. Membrane Proteins4 min

13.1.2. Practice: Membrane Proteins

13.2.1. Bacteriorhodopsin4 min

13.2.2. Porins2 min

13.2.3. Example: Bacteriorhodopsin1 min

13.2.4. Example: Membrane Proteins1 min

13.3.1. Introduction and Passive Transport7 min

13.3.2. Active Transport7 min

13.3.3. Transport Proteins: Ion Channels4 min

13.3.4. Example: Active vs. Passive Transport5 min

13.3.5. Practice: Potassium Ion Channels59 sec

13.4.1. Ion Channel Receptors2 min

13.4.2. G-Protein Coupled Receptors (GPCRs)7 min

13.4.3. Receptor Tyrosine Kinases (RTKs)5 min

13.4.4. Example: G-protein Coupled Receptors1 min

13.Q. Checkpoint Quiz4 Qns

14. Introduction to Metabolism and Thermodynamics

41min

14.1.1. Intro to Metabolism7 min

14.1.2. Gibbs Free Energy10 min

14.1.3. High Energy Intermediates9 min

14.1.4. Oxidation-Reduction Reactions6 min

14.1.5. Example: Oxidation-Reduction Reactions3 min

14.1.6. Example: Gibbs Free Energy3 min

14.1.7. Free energy of ATP52 sec

14.1.8. Coupled Reactions1 min

14.Q. Introduction to Metabolism4 Qns

15. Carbohydrate Structure

34min

15.1.1. Carbohydrates (Sugars)5 min

15.1.2. Carbohydrate Conformation and Annotation5 min

15.1.3. Carbohydrate Cyclization8 min

15.1.4. Example: Simple Sugars2 min

15.1.5. Example: Glucose2 min

15.1.6. Cheatsheet: Important Monosaccharides to Remember

15.2.1. Complex Carbohydrates7 min

15.2.2. Polysaccharides5 min

15.2.3. Example: Carbohydrate Structures1 min

15.Q. Checkpoint Quiz2 Qns

16. Carbohydrate Metabolism

19min

16.1.1. Glycolysis11 min

16.1.2. Glycolysis Regulation4 min

16.1.3. ATP production in Glycolysis1 min

16.1.4. Triose Phosphate Isomerase2 min

16.1.5. Glycolysis Regulation41 sec

16.1.6. Glycolysis: Cheatsheet

16.Q. Checkpoint Quiz4 Qns

17. Mitochondrial Bioenergetics: TCA Cycle

21min

17.1.1. Pyruvate Dehydrogenase6 min

17.1.2. Pyruvate Dehydrogenase Complex38 sec

17.1.3. Regulation of PDC1 min

17.2.1. Structure of Mitochondria3 min

17.2.2. Citric Acid Cycle8 min

17.2.3. The Citric Acid Cycle1 min

17.2.4. The Citric Acid Cycle49 sec

17.2.5. The Citric Acid Cycle: Cheatsheet

17.Q. Checkpoint Quiz5 Qns

18. Mitochondrial Bioenergetics: ETC

41min

18.1.1. Electron Transporters6 min

18.1.2. The Electron Transport Chain8 min

18.1.3. Electron Transport Inhibitors3 min

18.1.4. Electron Transporters49 sec

18.1.5. The electron transport chain1 min

18.1.6. Electron Transport Inhibitors42 sec

18.2.1. ATP and the Proton Motive Force (PMF)6 min

18.2.2. ATP Synthesis10 min

18.2.3. ATP production: Cheatsheet

18.3.1. Malate-Aspartate Shuttle5 min

18.Q. Checkpoint Quiz6 Qns

19. Fat Metabolism: Beta Oxidation

13min

19.1.1. Fat Degradation (Beta Oxidation)11 min

19.1.2. Fat Catabolism1 min

19.1.3. Beta-oxidation51 sec

19.Q. Checkpoint Quiz1 Qn

20. Fat Metabolism: Fatty Acid Synthesis

11min

20.1.1. Fat Synthesis10 min

20.1.2. Fatty Acid Synthesis2 min

20.Q. Checkpoint Quiz2 Qns

21. Gluconeogenesis

13min

21.1.1. Gluconeogenesis6 min

21.1.2. Regulating Gluconeogensis5 min

21.1.3. Pyruvate Kinase Regulation59 sec

21.1.4. Gluconeogenesis1 min

21.1.5. Glycolysis and Gluconeogenesis30 sec

21.Q. Checkpoint Quiz4 Qns

22. Ketogenesis

5min

22.1.1. Ketoneogenesis3 min

22.1.2. Ketoneogenesis47 sec

22.1.3. Ketoacidosis1 min

22.Q. Checkpoint Quiz2 Qns

Mock Exams

ME.1. Midterm - Mock Assessment7 Qns

ME.2. Final Exam - Mock Assessment72 Qns

ME.2 Final Exam - Mock Assessment

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