BIOCHEM 2280
UWO
Course Overview
Grade Boosters
Lessons & Practice
1. Fundamental concepts of biochemistry
18min2. Amino acids and ionization
1.3hr3. Protein structure and analysis
2.2hr3.7.1. Chromatography7 min3.7.2. Chromatography: Part II7 min3.7.3. SDS-PAGE (Electrophoresis)6 min3.7.4. Mass Spectrometry2 min3.7.5. X-ray Crystallography2 min3.7.6. Nuclear magnetic resonance (NMR)4 min3.7.7. Example: Determining Structure of Small Protein1 min3.7.8. Ammonium Sulfate Precipitation6 min3.7.9. Specific Activity1 min3.7.10. Activity Assays7 min
4. Protein function
56min4.4.1. Enzyme Classes7 min4.4.2. Active Site3 min4.4.3. Enzyme Mechanisms7 min4.4.4. Cofactors/Coenzymes2 min4.4.5. Chymotrypsin7 min4.4.6. Example: Enzyme Classes58 sec4.4.7. Example: Enzyme Mechanics1 min4.4.8. Example: Coenzymes and Cofactors33 sec4.4.9. Practice: Enzyme Catalyzed Reactions1 min4.4.10. Chymotrypsin2 min
5. Enzyme catalysis
27min6. Lipids and membranes
1.5hr6.1.1. Introduction to Lipids2 min6.1.2. Fatty Acids7 min6.1.3. Triacylglycerols2 min6.1.4. Glycerophospholipids3 min6.1.5. Sphingolipids2 min6.1.6. Cholesterol3 min6.1.7. Signalling Lipids (i.e., Steroids)3 min6.1.8. Example: Naming Fatty Acids3 min6.1.9. Example: Identifying Lipids1 min6.1.10. Practice: Fatty Acids1 min
7. Carbohydrate Structure
36min7.1.1. Carbohydrates (Sugars)5 min7.1.2. Carbohydrate Conformation and Annotation5 min7.1.3. Carbohydrate Cyclization8 min7.1.4. Complex Carbohydrates7 min7.1.5. Polysaccharides5 min7.1.6. Example: Simple Sugars2 min7.1.7. Example: Glucose2 min7.1.8. Example: Carbohydrate Structures1 min7.1.9. Practice: Naming Carbohydrates1 min7.1.10. Cheatsheet: Important Monosaccharides to Remember
8. Fundamental Concepts in Metabolism
32min9. Carbohydrate metabolism
58min10. Citric Acid Cycle
20min11. Oxidative phosphorylation
33min11.1.1. Electron Transporters6 min11.1.2. The Electron Transport Chain8 min11.1.3. ATP and the Proton Motive Force (PMF)6 min11.1.4. ATP Synthesis10 min11.1.5. Electron Transporters49 sec11.1.6. The electron transport chain1 min11.1.7. Electron Transport Inhibitors42 sec11.1.8. ATP production: Cheatsheet
12. Lipid Metabolism
28min13. Summary of energy metabolism
10min14. Nucleic Acid Structure
31min15. DNA Replication
34min16. DNA Damage and Repair
17min17. Molecular Basis of Cancer
36min18. Prokaryotic Transcription
38min19. Eukaryotic Transcription
20min20. RNA Processing
24min21. Translation
1.1hr22. Recombinant DNA Technology and Sequencing Genomes
1.5hr22.1.1. (Agarose) Gel Electrophoresis 3 min22.1.2. Northern Blot2 min22.1.3. Southern Blot2 min22.1.4. Polymerase Chain Reaction10 min22.1.5. Cloning and DNA libraries17 min22.1.6. Microarrays 3 min22.1.7. DNA Fingerprinting6 min22.1.8. CRISPR [detailed]9 min22.1.9. Example: Hybridization4 min22.1.10. Example: Cloning 3 min22.1.11. Example: DNA Transformation4 min22.1.12. Example: DNA Fingerprinting3 min
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