Biochemists can lyse thylakoid membranes into fragments, which can fold on them…

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Biochemists can lyse thylakoid membranes into fragments, which can fold on themselves to form vesicles. The vesicles can be either inside-out or right-side-out. Suppose you isolated a vesicle and found that the pH outside of the vesicle was very high and the pH inside the vesicle was low. What does that tell you about the orientation of the vesicle?
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