BIOCHEM 2280A
UWO
Course Overview
Grade Boosters
Lessons & Practice
1. Fundamental concepts of biochemistry
18min2. Amino acids and ionization
1.3hr3. Protein structure and analysis
2.2hr3.7.1. Chromatography3.7.2. Chromatography: Part II3.7.3. SDS-PAGE (Electrophoresis)3.7.4. Mass Spectrometry3.7.5. X-ray Crystallography3.7.6. Nuclear magnetic resonance (NMR)3.7.7. Example: Determining Structure of Small Protein3.7.8. Ammonium Sulfate Precipitation3.7.9. Specific Activity3.7.10. Activity Assays
4. Protein function
56min5. Enzyme catalysis
27min6. Lipids and membranes
1.5hr7. Carbohydrate Structure
36min7.1.1. Carbohydrates (Sugars)7.1.2. Carbohydrate Conformation and Annotation7.1.3. Carbohydrate Cyclization7.1.4. Complex Carbohydrates7.1.5. Polysaccharides7.1.6. Example: Simple Sugars7.1.7. Example: Glucose7.1.8. Example: Carbohydrate Structures7.1.9. Practice: Naming Carbohydrates7.1.10. Cheatsheet: Important Monosaccharides to Remember
8. Fundamental Concepts in Metabolism
32min9. Carbohydrate metabolism
58min10. Citric Acid Cycle
20min11. Oxidative phosphorylation
33min12. Lipid Metabolism
28min13. Summary of energy metabolism
10min14. Nucleic Acid Structure
31min15. DNA Replication
34min16. DNA Damage and Repair
17min17. Molecular Basis of Cancer
36min18. Prokaryotic Transcription
38min19. Eukaryotic Transcription
20min20. RNA Processing
24min21. Translation
1.1hr22. Recombinant DNA Technology and Sequencing Genomes
1.5hr22.1.1. (Agarose) Gel Electrophoresis 22.1.2. Northern Blot22.1.3. Southern Blot22.1.4. Polymerase Chain Reaction22.1.5. Cloning and DNA libraries22.1.6. Microarrays 22.1.7. DNA Fingerprinting22.1.8. CRISPR [detailed]22.1.9. Example: Hybridization22.1.10. Example: Cloning 22.1.11. Example: DNA Transformation22.1.12. Example: DNA Fingerprinting
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