BCHM 102
Queen's
Course Overview
Lessons & Practice
1. Water, Weak Bonds, and the Generation of Order Out of Chaos
46min2. Amino Acids
1.1hr3. Protein Three-Dimensional Structure
1.4hr4. Techniques in Protein Biochemistry
31min5. Structural Determination of Proteins
1hr5.2.1. X-ray Crystallography2 min5.2.2. Electron Microscopy2 min5.2.3. Nuclear magnetic resonance (NMR)4 min5.2.4. Infrared spectroscopy (IR)1 min5.2.5. Circular Dichroism (CD)3 min5.2.6. Example: Methods for Determining Protein Structure45 sec5.2.7. Example: Determining Structure of Small Protein1 min5.2.8. Practice: Match the Protein to the Technique2 min5.2.9. Example: Affinity Tag Chromatography2 min5.2.10. Example: Gel Filtration Chromatography2 min5.2.11. Chromatography: Part II7 min5.2.12. SDS-PAGE (Electrophoresis)6 min5.2.13. Chromatography7 min5.2.14. Mass Spectrometry2 min
6. Basic Concepts and Kinetics of Enzymes
41min6.2.1. Enzyme Classes7 min6.2.2. Active Site3 min6.2.3. Enzyme Mechanisms7 min6.2.4. Cofactors/Coenzymes2 min6.2.5. Chymotrypsin7 min6.2.6. Example: Enzyme Classes58 sec6.2.7. Example: Enzyme Mechanics1 min6.2.8. Example: Coenzymes and Cofactors33 sec6.2.9. Practice: Enzyme Catalyzed Reactions1 min6.2.10. Chymotrypsin2 min
7. Genetic Engineering of Proteins
38min8. Enzyme Assay (cont. Protein Purification and Identification)
14min9. Lipids and Biological Membranes
56min9.1.1. Introduction to Lipids2 min9.1.2. Fatty Acids7 min9.1.3. Triacylglycerols2 min9.1.4. Glycerophospholipids3 min9.1.5. Sphingolipids2 min9.1.6. Cholesterol3 min9.1.7. Signalling Lipids (i.e., Steroids)3 min9.1.8. Example: Naming Fatty Acids3 min9.1.9. Example: Identifying Lipids1 min9.1.10. Practice: Fatty Acids1 min
10. Signalling
27min10.1.1. Cell Signaling Overview3 min10.1.2. Types of ligands1 min10.1.3. Types of receptors1 min10.1.4. Secondary Messengers2 min10.1.5. GPCRs7 min10.1.6. Receptor Tyrosine Kinase5 min10.1.7. Signalling Lipids3 min10.1.8. Practice: Secondary messenger2 min10.1.9. Practice: G-Protein Coupled Receptors10.1.10. Practice2: G-Protein Coupled Receptors10.1.11. Practice3: G-Protein Coupled Receptors10.1.12. Practice: Receptor tyrosine kinases10.1.13. Practice2: Receptor tyrosine kinases3 min10.1.14. Practice3: Receptor tyrosine kinases
11. Carbohydrate Metabolism
2.1hr11.8.1. Electron Transporters6 min11.8.2. The Electron Transport Chain8 min11.8.3. Electron Transport Inhibitors3 min11.8.4. ATP and the Proton Motive Force (PMF)6 min11.8.5. ATP Synthesis10 min11.8.6. Electron Transporters49 sec11.8.7. The electron transport chain1 min11.8.8. Electron Transport Inhibitors42 sec11.8.9. ATP production: Cheatsheet
12. Photosynthesis
42min13. Fat metabolism, Lipoproteins & Ketogenesis
47min14. DNA Replication and Repair
54min15. Prokaryotic Transcription
42min16. Cancer Biology
36min17. Coordination of Metabolism
10min18. Intermolecular Forces + Properties of Water
46min19. Translation
1.1hr20. Recombinant DNA and Sequencing
1.6hr20.1.1. (Agarose) Gel Electrophoresis 3 min20.1.2. Northern Blot2 min20.1.3. Southern Blot2 min20.1.4. Polymerase Chain Reaction10 min20.1.5. Cloning and DNA libraries17 min20.1.6. DNA Sequencing9 min20.1.7. Microarrays 3 min20.1.8. DNA Fingerprinting6 min20.1.9. CRISPR [detailed]9 min20.1.10. Example: Hybridization4 min20.1.11. Example: Cloning 3 min20.1.12. Example: DNA Transformation4 min20.1.13. Example: DNA Fingerprinting3 min
21. Buffers
20min22. Enzyme Catalysis
27min23. Carbohydrate Structure
36min23.1.1. Carbohydrates (Sugars)5 min23.1.2. Carbohydrate Conformation and Annotation5 min23.1.3. Carbohydrate Cyclization8 min23.1.4. Complex Carbohydrates7 min23.1.5. Polysaccharides5 min23.1.6. Example: Simple Sugars2 min23.1.7. Example: Glucose2 min23.1.8. Example: Carbohydrate Structures1 min23.1.9. Practice: Naming Carbohydrates1 min23.1.10. Cheatsheet: Important Monosaccharides to Remember
24. Nucleic Acid Structure + DNA Denaturation
40min25. Fundamental Concepts of Biochemistry
18min26. Membrane Protein & Transporters
1.3hr26.1.1. Proteins in the Lipid Bilayer1 min26.1.2. Peripheral Membrane Proteins5 min26.1.3. Integral Membrane Proteins5 min26.1.4. Measuring Hydrophobicity7 min26.1.5. Membrane Protein Folding2 min26.1.6. Practice: Peripheral Membrane Proteins1 min26.1.7. Practice: Integral Membrane Proteins1 min26.1.8. Practice: Hydropathy49 sec26.1.9. Practice: Integral Membrane Proteins55 sec
