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Imagine you are a microbiologist growing different strains of bacteria in your …

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Wize University Biology Textbook > Lab Techniques for Biology

Plasmids & Restriction Enzymes

3 Activities

Imagine you are a microbiologist growing different strains of bacteria in your lab. You
have the following flasks:

  • i. Flask 1 contains strain A and no antibiotics
  • ii. Flask 2 contains strain A and antibiotics targeted for strain A
  • iii. Flask 3 contains strain A and strain B, and a wide spectrum antibiotic that targets all bacterial strains

In which flask is strain A mostly likely to become resistant to antibiotics
More Plasmids & Restriction Enzymes Questions:
What is the main disadvantage of using only one blunt end restriction enzyme in cloning?
Suppose you want to clone a certain gene of interest into a vector as shown below. Which restriction enzyme(s) should you use? Which restriction enzyme sites will be removed from the vector's polylinker?
Plasmid
You are attempting to construct a restriction map for a plasmid. You digest the plasmid with the restriction enzymes EcoR1 and Bbs1 and run the fragments through gel electrophoresis. Given the following gel results, which if the correct restriction map?
You are attempting to construct a restriction map for a plasmid. You digest the plasmid with the restriction enzymes EcoR1 and Bbs1 and run the fragments through gel electrophoresis. Given the following gel results, which if the correct restriction map?
In Homolgous Recombination in diploid yeast cells, why is it important to replace one copy of the gene you are studying with a gene for a selectable marker?
Plasmid
Cloning Plasmids
What is NOT a common feature found in in every plasmid?
You are attempting to construct a restriction map for a plasmid. You digest the plasmid with the restriction enzymes EcoR1 and Bbs1 and run the fragments through gel electrophoresis. Given the following gel results, which if the correct restriction map?
Imagine you want to insert a foreign gene into a plasmid to over express this gene. In order to do this successfully, what must both the plasmid and your gene have?
You are studying an olfactory receptor in two strains the roundworm C. elegans. In the wildtype strain, the worms are able to smell attractive food odors. In the mutant strain, the worms are unable to sense when food is nearby. You believe that a severe DNA mutation has led to a deletion of the olfactory receptor gene from the mutant C. elegans worm's genome. How would you detect whether this specific DNA sequence is present in the mutant C. elegans genome?
Cloning Question
Suppose you want to clone a certain gene of interest into a vector as shown below. Which restriction enzyme(s) should you use? Which restriction enzyme sites will be removed from the vector's polylinker?
Which of the following statements is FALSE regarding genomic DNA libraries?
What is the main disadvantage of using only one blunt end restriction enzyme in cloning?
Suppose you want to clone a certain gene of interest into a vector as shown below. Which restriction enzyme(s) should you use? Which restriction enzyme sites will be removed from the vector's polylinker?