Glycolysis: Important Takeaways

Glycolysis Full Sequence of Reactions and Summary

• Three irreversible steps:
• 1. Glucose to glucose 6-phosphate
• 2. Fructose 1,6-biphosphate
• 3. Phosphoenolpyruvate (PEP) to pyruvate
• ATP Balance:
• Two ATP consumed in the first half
• Two ATP produced in the second half x 2 = 4 ATP
• NADH, H+ produced x 2 = 2 NADH, H+
• Three highly regulated enzymes:
• Hexokinase
• Phosphofructokinase ("pacemaker enzyme")
• Pyruvate kinase
• Occurs in the cytoplasm.