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Which of the following would you need to change from the DNA sequence of this g…

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Wize University Biology Textbook > Transcription and Translation

Prokaryotic Transcription

6 Activities

You want to express the protein for epidermal growth factor in a bacterial cell. Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed?
  1. Remove introns
  2. Remove the SD box
  3. Add a terminator sequence
  4. Add the poly-A-tail
More Prokaryotic Transcription Questions:
Compare prokaryotic and eukaryotic transcription. Where possible, try to explain why these differences exist.
Transcription Initiation
What is the DNA sequence to which RNA polymerse binds and transcription starts?
Prokaryotic transcription differs from eukaryotic transcription in that... (select all that apply)
Transcription Termination
Which of the following are not involved in transcription termination?
Practice: Eukaryotic vs. Prokaryotic Transcription
Which of the following is incorrect regarding the differences between prokaryotic and eukaryotic transcription?
In
prokaryotic
cells, mRNA is transcribed directly from the DNA template without additional processing.
Practice: Eukaryotic vs. Prokaryotic Transcription
Which of the following is incorrect regarding the differences between prokaryotic and eukaryotic transcription?
How does prokaryotic transcription begin?
In eukaryotes, which of the following is true about transcription:
i. Removal of exons
ii. Addition of a poly-U tail
What is the base sequence of the mRNA strand that would be synthesized from the following DNA template strand?
5'-GTTAAC-3'
If there was a mutation in the DNA sequence at the -35 box in prokaryotes what phases of transcription do you think would be effected and why?
Bonus: which subunit binds to the -35 box?
Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed? - Practice Question
You want to express the protein for epidermal growth factor in a bacterial cell. Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed?
The synthesis, processing and function of a eukaryotic miRNA involves complimentary base pairing with which of the following?
DNA
tRNA
Which of the following information is understood by the cell in the form of RNA?
Consider a typical prokaryotic gene as shown. Which of the numbers indicates a sequence that is transcribed but not translated?
Why is annotating a eukaryotic genome a lot more difficult than a prokaryotic genome?
You want to compare distantly related organisms from eukaryotes to bacteria. Which of the following would you use?
Consider a gene that has moved through lateral gene transfer from the mitochondria to the nucleus. This gene, although now in the nucleus, still exerts its function in the mitochondria. What would this gene absolutely need to acquire in order to be properly expressed?
What is required before the elongation of a polypeptide in a prokaryotic cell can begin?
Which of the following is understood by the prokaryotic cell as RNA?
What is the correct order of structural elements in a prokaryotic gene?
Which of the following are understood by the prokaryotic cell as RNA?
A heat shock protein increases in abundance after exposure to 40°C compared to 24°C. You can visually see the increase in band intensity on a Western blot. You treat your cells with MG132 to block the activity of the proteasome as well as treating the cells with antinomycin to block transcription. Looking at your Western blot, the protein abundance of the heat shock protein decreases over time at the same temperature. What is the most likely reason for this observed decrease?
In
prokaryotic
cells, mRNA is transcribed directly from the DNA template without additional processing.
Transcription Question
Which of the following enzymes is used in the elongation phase of transcription?
Prokaryotic transcription differs from eukaryotic transcription in that... (select all that apply)
Practice: Eukaryotic vs. Prokaryotic Transcription
Which of the following is incorrect regarding the differences between prokaryotic and eukaryotic transcription?
Which of the following is false regarding transcription?
Draw a prokaryotic gene and its RNA product.
Transcription Termination
Which of the following are not involved in transcription termination?
Transcription Initiation
What is the DNA sequence to which RNA polymerse binds and transcription starts?
Compare prokaryotic and eukaryotic transcription. Where possible, try to explain why these differences exist.