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A heat shock protein increases in abundance after exposure to 40°C compared to …

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Wize University Biology Textbook > Transcription and Translation

Prokaryotic Transcription

6 Activities

Wize University Biology Textbook > Lab Techniques for Biology

Nucleic Acid Detection [Northern and Southern Blots]

3 Activities

Wize University Biology Textbook > Gene Expression & Regulation

Introduction to Regulation of Gene Expression

7 Activities

A heat shock protein increases in abundance after exposure to 40°C compared to 24°C. You can visually see the increase in band intensity on a Western blot. You treat your cells with MG132 to block the activity of the proteasome as well as treating the cells with antinomycin to block transcription. Looking at your Western blot, the protein abundance of the heat shock protein decreases over time at the same temperature. What is the most likely reason for this observed decrease?
More Prokaryotic Transcription Questions:
Compare prokaryotic and eukaryotic transcription. Where possible, try to explain why these differences exist.
Transcription Initiation
What is the DNA sequence to which RNA polymerse binds and transcription starts?
Prokaryotic transcription differs from eukaryotic transcription in that... (select all that apply)
Transcription Termination
Which of the following are not involved in transcription termination?
Practice: Eukaryotic vs. Prokaryotic Transcription
Which of the following is incorrect regarding the differences between prokaryotic and eukaryotic transcription?
In
prokaryotic
cells, mRNA is transcribed directly from the DNA template without additional processing.
Practice: Eukaryotic vs. Prokaryotic Transcription
Which of the following is incorrect regarding the differences between prokaryotic and eukaryotic transcription?
Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed? - Practice Question
You want to express the protein for epidermal growth factor in a bacterial cell. Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed?
Remove introns
Remove the SD box
How does prokaryotic transcription begin?
In eukaryotes, which of the following is true about transcription:
i. Removal of exons
ii. Addition of a poly-U tail
What is the base sequence of the mRNA strand that would be synthesized from the following DNA template strand?
5'-GTTAAC-3'
If there was a mutation in the DNA sequence at the -35 box in prokaryotes what phases of transcription do you think would be effected and why?
Bonus: which subunit binds to the -35 box?
Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed? - Practice Question
You want to express the protein for epidermal growth factor in a bacterial cell. Which of the following would you need to change from the DNA sequence of this gene in order to have it be properly expressed?
The synthesis, processing and function of a eukaryotic miRNA involves complimentary base pairing with which of the following?
DNA
tRNA
Which of the following information is understood by the cell in the form of RNA?
Consider a typical prokaryotic gene as shown. Which of the numbers indicates a sequence that is transcribed but not translated?
Why is annotating a eukaryotic genome a lot more difficult than a prokaryotic genome?
You want to compare distantly related organisms from eukaryotes to bacteria. Which of the following would you use?
Consider a gene that has moved through lateral gene transfer from the mitochondria to the nucleus. This gene, although now in the nucleus, still exerts its function in the mitochondria. What would this gene absolutely need to acquire in order to be properly expressed?
What is required before the elongation of a polypeptide in a prokaryotic cell can begin?
Which of the following is understood by the prokaryotic cell as RNA?
What is the correct order of structural elements in a prokaryotic gene?
Which of the following are understood by the prokaryotic cell as RNA?
In
prokaryotic
cells, mRNA is transcribed directly from the DNA template without additional processing.
Transcription Question
Which of the following enzymes is used in the elongation phase of transcription?
Prokaryotic transcription differs from eukaryotic transcription in that... (select all that apply)
Practice: Eukaryotic vs. Prokaryotic Transcription
Which of the following is incorrect regarding the differences between prokaryotic and eukaryotic transcription?
Which of the following is false regarding transcription?
Draw a prokaryotic gene and its RNA product.
Transcription Termination
Which of the following are not involved in transcription termination?
Transcription Initiation
What is the DNA sequence to which RNA polymerse binds and transcription starts?
Compare prokaryotic and eukaryotic transcription. Where possible, try to explain why these differences exist.
More Nucleic Acid Detection [Northern and Southern Blots] Questions:
Practice: Gene Expression
Which technique would allow you to determine whether the transcription from the CFTR gene was compromised in samples obtained from patients with cyctic fibrosis?
Which technique would allow you to determine whether the transcription from the CFTR gene, was compromised in samples obtained from patients with cyctic fibrosis?
Interpreting and drawing Northern blots
Using the following Northern blot, what can we conclude about the gene expression of Hsp1 in high temperatures? Fill in what you predict to be the banding pattern of Hsp1 and 5S RNA levels at 20oC.
You want to measure the rate of Hsp1 mRNA decay at 55oC. Design an experiment to answer that question using a Northern blot as your read-out.
Practice: Gene Expression
Which technique would allow you to determine whether the transcription from the CFTR gene was compromised in samples obtained from patients with cyctic fibrosis?
Which of the following can be resolved using Western blotting?
1. Protein-protein interaction
2. Expression of a protein when induced with a drug
Use the following SDS-PAGE and Western Blot results for this 2 part question:
You are running an experiment where you want to determine if in a eukaryotic cell the rate of an mRNA transcript being degraded. Which of the following describes the experiment and technique you will use?
Unicellular organisms have the ability to change their membrane fluidity depending on temperature. A bacteria is able to survive at -10oC. At this temperature, what would you expect to see on a Northern blot probing for desaturase?
Interpreting and drawing Western blots
Using the following Western blot, what can we conclude about the protein abundance of HSP1?
What would you add to the preceding experiment to measure the rate of HSP1 degradation?
What would you add to the preceding experiment to measure the rate of HSP1 translation?
Interpreting and drawing Northern blots
Using the following Northern blot, what can we conclude about the gene expression of Hsp1 in high temperatures? Fill in what you predict to be the banding pattern of Hsp1 and 5S RNA levels at 20oC.
You want to measure the rate of Hsp1 mRNA decay at 55oC. Design an experiment to answer that question using a Northern blot as your read-out.
You are studying an olfactory receptor in two strains the roundworm C. elegans. In the wildtype strain, the worms are able to smell attractive food odors. In the mutant strain, the worms are unable to sense when food is nearby. You believe that a severe DNA mutation has led to a deletion of the olfactory receptor gene from the mutant C. elegans worm's genome. How would you detect whether this specific DNA sequence is present in the mutant C. elegans genome?
Which technique would allow you to determine whether the transcription from the CFTR gene, was compromised in samples obtained from patients with cyctic fibrosis?
Which technique would allow you to determine whether the transcription from the CFTR gene, was compromised in samples obtained from patients with cyctic fibrosis?
You are studying an olfactory receptor in two strains the roundworm C. elegans. In the wildtype strain, the worms are able to smell attractive food odors. In the mutant strain, the worms are unable to sense when food is nearby. You believe that a severe DNA mutation has led to a deletion of the olfactory receptor gene from the mutant C. elegans worm's genome. How would you detect whether this specific DNA sequence is present in the mutant C. elegans genome?
In Northern blotting, electrophoresis is used to resolve which biological molecules? What type of probe is used to identify the target molecule(s)?
Source of this question: Griffiths AJ, et al. An introduction to genetic analysis. 11th Ed.
More Introduction to Regulation of Gene Expression Questions:
For any gene, high rates of transcription don’t necessarily lead to high rates of translation. Why not?
Which of the following accurately describes why a single SNP could affect the expression of more than one protein in eukaryotes?
For any gene, high rates of transcription don’t necessarily lead to high rates of translation. Why not?
Which of the following is true regarding prokaryotic operons?
You have isolated a novel miRNA (miR-007) that affects the development of ears in rabbits.
Loss if MiR-007 leads to a mutant phenotype in which the positioning of the ears is dorsal (wild-type have frontal positioning), and the ears were significantly smaller than the regular size. With further investigations you find that transcription factor (P007) is increased in the mutant type, while the levels of mRNA are the same compared to the wild-type. The levels of proteins have changed.
What type of regulatory protein is P007?
Use the following diagram to answer the true or false questions below.
The two genes are in an operon.
A single operon containing 5 genes has how many promoter regions?
Practice: Gene Mechanisms
You are doing an experiment to determine the mechanism of gene
expression of a bacterial operon responsible for the utilization of glycerol. You know Abc1 is the regulator. You have two strains of the bacteria: one has the normal Abc1 regulator (+abc1), and the other does not (-abc1). You obtain the following results when growing the two strains with and without glycerol:
Describe the mechanism of regulation.