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Practice: Gene Mechanisms

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Wize University Biology Textbook > Gene Expression & Regulation

Eukaryotic Transcriptional Regulation [Transcription Factors, Enhancers and Repressors]

3 Activities

Wize University Biology Textbook > Gene Expression & Regulation

Eukaryotic Translational/Post-Translational Regulation

2 Activities

Wize University Biology Textbook > Gene Expression & Regulation

Introduction to Regulation of Gene Expression

7 Activities

You are doing an experiment to determine the mechanism of gene
expression of a bacterial operon responsible for the utilization of glycerol. You know Abc1 is the regulator. You have two strains of the bacteria: one has the normal Abc1 regulator (+abc1), and the other does not (-abc1). You obtain the following results when growing the two strains with and without glycerol:

Describe the mechanism of regulation.
More Eukaryotic Transcriptional Regulation [Transcription Factors, Enhancers and Repressors] Questions:
Why is annotating a eukaryotic genome a lot more difficult than a prokaryotic genome?
The human body often expresses different proteins or amounts of proteins originating from the same gene in different tissues. Which of the following are possible explanations for this phenomena?
Which of the following proteins would you find bound to DNA during eukaryotic gene expression?
Which of the following can regulate the initiation of transcription?
Write a point form essay outline for the following prompt:
The central dogma of molecular biology is that DNA is transcribed to RNA, which is translated into protein. As such, when we think of DNA, we often picture transcriptionally active euchromatin. However, approx. 10% of interphase DNA in eukaryotes is organized as transcriptionally inactive heterochromatin. Explain the importance of heterochromatin.
Thesis statement:
The retinoblastoma (Rb) protein is named after a rare form of cancer that affects the retina (the light-detecting tissue of the eye). In patients with this type of cancer, what do you think has happened to the Rb protein?
More Eukaryotic Translational/Post-Translational Regulation Questions:
Which of the following is false about phosphorylation as a molecular switch (activates proteins)?
Phosphorylation is a type of molecular switch that occurs at what level?
Practice: Transcription & Translation
Both transcription and translation are said to proceed via three steps: initiation, elongation, and termination. Focusing on eukaryotes, summarize each step for transcription and translation.
More Introduction to Regulation of Gene Expression Questions:
For any gene, high rates of transcription don’t necessarily lead to high rates of translation. Why not?
Which of the following accurately describes why a single SNP could affect the expression of more than one protein in eukaryotes?
For any gene, high rates of transcription don’t necessarily lead to high rates of translation. Why not?
Which of the following is true regarding prokaryotic operons?
A heat shock protein increases in abundance after exposure to 40°C compared to 24°C. You can visually see the increase in band intensity on a Western blot. You treat your cells with MG132 to block the activity of the proteasome as well as treating the cells with antinomycin to block transcription. Looking at your Western blot, the protein abundance of the heat shock protein decreases over time at the same temperature. What is the most likely reason for this observed decrease?
You have isolated a novel miRNA (miR-007) that affects the development of ears in rabbits.
Loss if MiR-007 leads to a mutant phenotype in which the positioning of the ears is dorsal (wild-type have frontal positioning), and the ears were significantly smaller than the regular size. With further investigations you find that transcription factor (P007) is increased in the mutant type, while the levels of mRNA are the same compared to the wild-type. The levels of proteins have changed.
What type of regulatory protein is P007?
Use the following diagram to answer the true or false questions below.
The two genes are in an operon.
A single operon containing 5 genes has how many promoter regions?