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Which of the following is true regarding prokaryotic operons?

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Wize University Biology Textbook > Gene Expression & Regulation

Prokaryotic Operons: Lactose (lac) Operon [detailed]

6 Activities

Wize University Biology Textbook > Gene Expression & Regulation

Introduction to Regulation of Gene Expression

7 Activities

Which of the following is true regarding prokaryotic operons?
More Prokaryotic Operons: Lactose (lac) Operon [detailed] Questions:
Which of the following are accurate statements describing a direct interaction between a nucleic acid sequence and a protein? - Practice Question
Which of the following are accurate statements describing a direct interaction between a nucleic acid sequence and a protein?
tRNA and the start codon
RNA polymerase and a gene promoter
What would be the phenotype of a bacterial cell with a loss of function mutation in the lac operon promoter?
If there was a mutant that showed constitutive expression of the lac operon, and there is still functional lac repressor protein present in the cell, what is the likely location of the mutation?
Which of the following accurately describe what happens when a bacterial cell is grown in the presence of lactose only?
How does CAP induce expression of the genes in the lac operon?
In which situation would the CAP protein be bound to the lac promoter?
You have a strain of E.coil and identify that it has a mutation is its lac operator sequence such that the lac repressor can no longer bind. What would you expect would happen to the level of B-galactosidase in the cell if lactose is absent and glucose in present?
You discover a mutant strain of E.coli such that it cannot grow when lactose is present in the environment. Where might this mutation be?
Which of the following are accurate statements describing a direct interaction between a nucleic acid sequence and a protein? - Practice Question
Which of the following are accurate statements describing a direct interaction between a nucleic acid sequence and a protein?
In bacteria, a repressor protein binds to the lac operon when cells are grown in the presence of...
Lac Operon
The following are the genotypes of partial diploid bacteria, mutant in one or more genes associated with the LAC OPERON. For each bacterium, choose from the choices provided whether beta-galactosidase and whether permease will be produced under conditions when lactose is present and when lactose is absent in accordance to the table below.
Lac Operon
The following are the genotypes of partial diploid bacteria, mutant in one or more genes associated with the LAC OPERON. For each bacterium, choose from the choices provided whether beta-galactosidase and whether permease will be produced under conditions when lactose is present and when lactose is absent in accordance to the table below.
The following are the genotypes of partial diploid bacteria, mutant in one or more genes associated with the LAC OPERON. For each bacterium, indicate in the spaces provided whether beta-galactosidase and whether permease will be produced under conditions when lactose is present and when lactose is absent. Use "yes" to indicate that the enzyme is produced (in functional form); use "no" to indicate that the enzyme either is not produced or is not functional.
For the following table, predict the expression parent on the respective enzymes. Use the following symbols: " - " no protein product, " + " some protein product, " +++ " a lot of proteins produced.
The following are the genotypes of partial diploid bacteria, mutant in one or more genes associated with the LAC OPERON. For each bacterium, choose from the choices provided whether beta-galactosidase and whether permease will be produced under conditions when lactose is present and when lactose is absent in accordance to the table below.
The following are the genotypes of partial diploid bacteria, mutant in one or more genes associated with the LAC OPERON. For each bacterium, choose from the choices provided whether beta-galactosidase and whether permease will be produced under conditions when lactose is present and when lactose is absent in accordance to the table below.
More Introduction to Regulation of Gene Expression Questions:
For any gene, high rates of transcription don’t necessarily lead to high rates of translation. Why not?
Which of the following accurately describes why a single SNP could affect the expression of more than one protein in eukaryotes?
For any gene, high rates of transcription don’t necessarily lead to high rates of translation. Why not?
A heat shock protein increases in abundance after exposure to 40°C compared to 24°C. You can visually see the increase in band intensity on a Western blot. You treat your cells with MG132 to block the activity of the proteasome as well as treating the cells with antinomycin to block transcription. Looking at your Western blot, the protein abundance of the heat shock protein decreases over time at the same temperature. What is the most likely reason for this observed decrease?
You have isolated a novel miRNA (miR-007) that affects the development of ears in rabbits.
Loss if MiR-007 leads to a mutant phenotype in which the positioning of the ears is dorsal (wild-type have frontal positioning), and the ears were significantly smaller than the regular size. With further investigations you find that transcription factor (P007) is increased in the mutant type, while the levels of mRNA are the same compared to the wild-type. The levels of proteins have changed.
What type of regulatory protein is P007?
Use the following diagram to answer the true or false questions below.
The two genes are in an operon.
A single operon containing 5 genes has how many promoter regions?
Practice: Gene Mechanisms
You are doing an experiment to determine the mechanism of gene
expression of a bacterial operon responsible for the utilization of glycerol. You know Abc1 is the regulator. You have two strains of the bacteria: one has the normal Abc1 regulator (+abc1), and the other does not (-abc1). You obtain the following results when growing the two strains with and without glycerol:
Describe the mechanism of regulation.