Wize University Biochemistry Textbook > Structural Determination of Proteins

Additional Practice

Practice: Protein Structure Analysis in the Presence of Denaturant

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Queen's University

In the presence of Urea a protein completely unfolds, but regenerates upon dialysis of the urea. Using IR, CD, and Fluorescence you monitor the protein. which statement is FALSE about this scenario?

In the presence of urea there will be drastic changes in the CD and IR spectra compared to wild type

Upon denaturation in urea, Fluorescence will no longer occur

CD and IR will be able to identify regeneration of secondary structure upon dialysis

Fluorescence can monitor the protein via its tryptophan residues

None of the above

I don't know