Genomic Mapping

Gene Mapping

• Gene mapping starts with linkage analysis to determine which genes are close together (linked).
• Different alleles of the same gene give different phenotypes.
• The different alleles have a different DNA sequence.
• Gene mapping can be done to determine which allele gives a particular phenotype.

• Single nucleotide polymorphisms (SNPs) are single base pair changes in the gene.
• Restriction fragment length polymorphisms (RFLPs) occur when the genome is digested with restriction enzymes, and a SNP that occurs in the specific restriction site so that the restriction enzyme can no longer cut at that particular location.
• This will result in a different band pattern on an electrophoresis gel.