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The CRISPR/Cas9 system and RNA interference by siRNA are both methods used to k…

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Wize University Biology Textbook > Lab Techniques for Biology

Gene Editing

4 Activities

Wize University Biology Textbook > Gene Expression & Regulation

Eukaryotic Post-Transcriptional Regulation

3 Activities

The CRISPR/Cas9 system and RNA interference by siRNA are both methods used to knockdown proteins. Which of the following is NOT a key difference between the two techniques?
More Gene Editing Questions:
What is the phenotype of a CED-4 knockout in C. elegans - Practice Question
Using CRISPR you have knocked out the CED-4 gene in the nematode C. elegans. Which of the following statements are TRUE? (Select all that apply)
O a. In the absence of death signal apoptosis would be activated
O b. In the presence of death signal apoptosis would not be activated
What is the phenotype of a CED-4 knockout in C. elegans - Practice Question
Using CRISPR you have knocked out the CED-4 gene in the nematode C. elegans. Which of the following statements are TRUE?
The CRISPR/Cas9 system and RNA interference by siRNA are both methods used to knockdown proteins. Which of the following is NOT a key difference between the two techniques?
What type of enzyme activity does the Cas protein have?
The CRISPR/CAS9 system is an adaptation of which natural phenomenon?
Genome Editing
Explain the differences and similarities between TALENs and Zinc Finger Nucleases.
CRISPR
What does CRISPR stand for?
Genome Editing
Which statement is FALSE about genomic editing techniques?
More Eukaryotic Post-Transcriptional Regulation Questions:
You are studying two distinct protein isoforms which seem to arise from the same gene. The two isoforms seem to arise due to a single base pair change found on the mRNA transcript that changes the C at position 854 on the mRNA to a U. As a result of this change, isoform 1 contains the codon CAG (Glutamine) and is translated into a full length protein. However, isoform 2 contains the codon UAG (Stop) and is translated into a truncated protein. This change is most likely the result of which of the following?
There is a gene that codes for a protein that is differentially expressed in lung and heart tissue. What are some possible ways that this gene is post-transcriptionally regulated?
Which of the following accurately describes why a single SNP could affect the expression of more than one protein in eukaryotes?
Which of the following are neither transcribed nor translated?
TATA box
Enhancer
What is the mechanism by which microRNA affects the production of gene products?
The human body often expresses different proteins or amounts of proteins originating from the same gene in different tissues. Which of the following are possible explanations for this phenomena?
There is a gene that codes for a protein that is differentially expressed in lung and heart tissue. What are some possible ways that this gene is post-transcriptionally regulated?
The CRISPR/Cas9 system and RNA interference by siRNA are both methods used to knockdown proteins. Which of the following is NOT a key difference between the two techniques?
You are studying two distinct protein isoforms which seem to arise from the same gene. The two isoforms seem to arise due to a single base pair change found on the mRNA transcript that changes the C at position 854 on the mRNA to a U. As a result of this change, isoform 1 contains the codon CAG (Glutamine) and is translated into a full length protein. However, isoform 2 contains the codon UAG (Stop) and is translated into a truncated protein. This change is most likely the result of which of the following?
You are studying the mRNA concentration levels of a certain cytokine gene in a cell under different stressful conditions. You notice that when the cell is placed under a chemical stressor, the cytokine protein is present at very high amounts in the cells. Once the stressor is removed, cytokine proteins are almost completely gone. You also observe that in both the stressed and non-stressed conditions, the concentration of the cytokine transcript remains the same. What is the most likely explanation for this observation?
Practice: Eukaryotic Cells
Certain proteins are nearly universal amongst eukaryotic cells. However, when comparing different organisms, the sequences of the genes that encode these proteins can vary quite a bit.
Provide two explanations as to how this may be possible.