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The ribosome is made up of

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Wize University Biology Textbook > Transcription and Translation

Translation into Proteins

7 Activities

Wize University Biology Textbook > Cell Structure

The Endomembrane System

9 Activities

The ribosome is made up of

More Translation into Proteins Questions:
Match the correct options.
tRNA
Why are there so many tRNA molecules?
Which of the following statements is true regarding translation?
What type of interaction occurs between the codon of the mRNA and anticodon of the tRNA:
What end of the protein molecule is synthesized first?
What is the role of tRNA?
a) Read mRNA codons using anticodons and assemble amino acids in the proper sequence to make a protein
b) Package and transport proteins to various locations in the cell
Where do both transcription and translation take place in eukaryotes?
Ribosomes
What are ribosomes responsible for producing?
Practice: tRNA vs mRNA
An anticodon of a tRNA has the sequence 5'-GCA-3'. Use an amino acid table like the one from the theory to help you!
a) What amino acid does the tRNA carry?
b) What is a complimentary mRNA codon?
The ribosome preforms the process called _____ while RNA polymerase preforms the process called ____
Practice: Translation
Match the correct options.
Practice: Components for Protein Translation
Which of the following is not necessary for protein translation to occur?
Which of the following functions as an RNA-protein complex?
Ribosomes
snRNPs
If the codon for arginine in DNA is 3'GCA, what would be the expected tRNA anticodon sequence?
Consider a typical prokaryotic gene as shown. Which of the numbers indicates a sequence that is transcribed but not translated?
Why is annotating a eukaryotic genome a lot more difficult than a prokaryotic genome?
What is required before the elongation of a polypeptide in a prokaryotic cell can begin?
How is a finished polypeptide released from the ribosome?
Which of the following is not necessary for protein translation to occur?
What is synthesized at the N-termini of every polypeptide in eukaryotes?
What is the first amino acid of a translated polypeptide?
If a reading frame shift occurs during translation of an mRNA transcript, what is the MOST likely outcome?
Match the following enzymes with their function.
Translation and Primary structure Question
A protein coding polypeptide chain... (select all that apply)
Translation
Which of the following statements is true regarding translation?
Use the codon dictionary below to complete the following table. Assume that reading is from left to right and that the columns represent transcriptional and translational alignments.
Translation Initiation
How is translation initiation different between prokaryotes and eukaryotes?
tRNA
Why are there so many tRNA molecules?
Explain the stages of translation elongation including the ribosomal sites in your answer.
Match the correct options.
Membrane Proteins
If a random protein was mutated, what effect do you think that the following amino acid substitutions would have on the structure of the protein? Would these substitutions affect secondary, tertiary, or quaternary protein structure?
a. Cys →\rightarrow Pro
b. Asp →\rightarrowLys
More The Endomembrane System Questions:
Ribosome in nucleus
Where in the nucleus are ribosomes assembled? [answer is one word]
Your friend is trying to convince you that the ER was one a prokaryotic cell and was engulfed by a eukaryotic cell. He says they had a symbiotic relationship that allowed then to continue living on together. What evidence could you present to him that would disprove his theory?
How do water-soluble molecules move into the ER lumen?
What would happen if the a ribosome attached to the ER membrane created a transmembrane protein with a deformed STA hydrophobic region? Assume that the deformation involves the addition of some hydrophilic amino acids.
The inner membrane of the mitochondria is filled with cristae (folded parts). How would this aid in increasing ATP production? Explain the structure-function relationship of the inner mitochondrial membrane.
Which of the following things does not describe the cytoplasm
Similar to Midterm 1 2018
Explain how you could change the rate of fusion to make the mitochondria in a cell larger. Name 2 things you could do to make this happen.
Smooth ER
Which of the following best describes the smooth ER?
Hydrolytic enzymes
Where could you expect to find hydrolytic enzymes?
Practice: Cell Theory
Which of the following is a premise of cell theory?
Golgi
Which of the following is a function of the golgi apparatus?
Ribosome in nucleus
Where in the nucleus are ribosomes assembled? [answer is one word]
A desmosome connects cells of one type of tissue together by...
All cells store their DNA in the nucleus and/or additionally in the mitochondria/chloroplast. T or F
Practice: Endomembrane 2
Predict what coat protein is lining the vesicles moving between these organelles?
1. Between B to C
2. Between C to B
If cadherin is a type I transmembrane protein, it's structure does NOT include:
If acetyltransferase is an ER-bound membrane protein where its N-terminus catalytic domain works in the ER lumen, what type of membrane protein is it?
Which of the following does not use clathrin coated vesicles?
Which of the following is most likely to occur if a glycosylation enzyme in the ER was mutated?
Which of the following statements about co-translation translocation into the ER is true?
1. Ribosomes are permanently bound to the ER surface
2. ER signal sequence is recognized by the SRP (signal recognition particle)
Which of the following experimental findings support the idea that secreted proteins are synthesized in the ER?
Signal peptide sequences exist in cargo protein to ensure that they are direct to the correct location along the endomembrane system. Which of the following statements correctly describes signal peptide sequences?
1. KDEL is the Golgi retention signal peptide sequence
2. Peptide sequences are recognized by the correct coat proteins
How many start ER signal sequences would you expect to find in the following transmembrane protein?
Considering the following ER transmembrane proteins, which of the following proteins would have their C-terminus inside the ER lumen? Select all that apply.
Use the following to answer this 3-part question:
Consider the 6 different ER membrane protein orientation to answer this 2 part question:
3 domains
Which of the following is a membrane-bound organelle? (select all that apply)
Where along the endomembrane system would you find COPI coat protein?
Practice: Endomembrane 1
Name the structures indicated on the figure below:
Where in the Chlamydomonas cell would you find mRNA with a 5’ CAP?
What is the role of the nucleolus?
In which of the following organelles is addition and modification of oligosaccharides to glycoproteins completed?
What is the function of nuclear pore complexes?
Which of the following describes exocytosis?
Turgor pressure is most closely linked to which of the following?
How are lysosomes maintained at such a low pH?
How do vesicles move about the cell?
Which of the following is not a function of the Golgi?
In what structure is glycosylation typically initiated?
Which of the following is not a function of the smooth ER?
What is the perinuclear space?
Fill in the blanks: Lysosomes are maintained at __________ pH through the action of
____________
What is the cis Golgi network (CGN) is thought of as?
Which of the following is a non-membranous structure?
Which of the following is not a function of the Golgi apparatus?
What is the function of nuclear pore complexes?
Where is the internal leaflet of an ER vesicle oriented after fusing with the Golgi?
Which of the following is false regarding lysosomes?
A peripheral membrane protein that was synthesized in the rough ER lumen would be facing which direction in a vesicle after Golgi sorting?
What is the Golgi generally responsible for?
The trans Golgi is considered ...
Where does protein modification typically start?
Which of the following is not included in the endomembrane system?
Where is the site of ribosomes biogenesis?
Put the following in order of size from LARGEST to SMALLEST:
Virus, nucleus, bacteria, eukaryotic cell, ribosome
Which of the following is not a function of the Golgi apparatus?
Which of the following is false regarding ribosomes?
Which of the following are part of the endomembrane system?
Botulinum toxin is a protein which contains a heavy chain and a light chain. The heavy chain guides the toxin to neurons, where the light chain is released into the cytoplasm. The light chain is a protein-degrading enzyme (protease) that targets SNARE proteins. Some people like to inject this toxin in order to relax their facial muscles ¯\_(ツ)_/¯. Explain how botulinum toxin might inhibit muscle contractions.
You are interested in studying a secretory protein (protein X). Propose an experiment that would allow you to determine whether it is secreted via constitutive exocytosis or regulated exocytosis.
Write a point form essay outline for the following prompt:
Trace the pathway by which a hydrolytic enzyme localizes to the lysosome. Explain where the
enzyme is synthesized, which endomembrane compartments it must go through, and how it is
Wize Concept Check
Endosomes are responsible for sorting endocytosed macromolecules. What are the possible outcomes for molecules sent to an endosome? For each possible outcome, give an example of a molecule that might be processed in that way.
Wize Concept Check
Glycosylation of proteins in the secretory pathway takes place over several steps, mediated by enzymes that are located in the ER and in different Golgi cisternae. Why do you think that these enzymes are separated into different compartments of the endomembrane system?
Wize Concept Check
Once a vesicle has budded from a membrane, it sheds its coat proteins (clathrin/COP proteins). What proteins remain on the vesicle surface? How does the vesicle recognize its target membrane?
Wize Concept Check
Explain the role of each of the following components in the formation of a clathrin-coated
vesicle:
a. Cargo receptor: